李双双1,2,赵 辉1,2*
(1.黑龙江大学 农业微生物技术教育部工程研究中心,黑龙江哈尔滨 150500;
2.黑龙江大学 生命科学学院 黑龙江省普通高校微生物重点实验室,黑龙江哈尔滨 150080)
摘要:阿魏酸具有抗氧化、降血脂、抑菌消炎等生理功能,阿魏酸酯酶可以使阿魏酸甲酯、低聚糖阿魏酸酯等以酯键结合的阿魏酸游离出来,同时将香豆酸等抗氧化活性物质水解释放,在食品及医药等行业具有很大的应用价值。本文对微生物发酵产阿魏酸酯酶及其理化性质、结构、分类进行了阐述,同时对不同来源的阿魏酸酯酶基因克隆及阿魏酸酯酶在食品和医药等行业的应用进行了综述。
关键词:微生物;阿魏酸酯酶;外源表达
中图分类号:Q815 文献标识码:A 文章编号:1674-506X(2020)05-0075-0006
Research Progress on the Properties and Applications of Microbial Ferulic Acid Esterase
LI Shuang-shuang1,2, ZHAO Hui1,2*
(1.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University,
Harbin Heilongjiang 150500, China;
2.Key laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin Heilongjiang 150080, China)
Abstract:Ferulic acid has physiological functions such as anti-oxidation, lowering blood fat, antibacterial and anti-inflammatory, and ferulic acid esterase can free the ferulic acid bound by ester bonds such as methyl ferulate and oligosaccharide ferulic acid ester. At the same time, it will release coumaric acid and other antioxidant active substances by hydrolysis, which has great application value in food and medicine industries. This article describes the production of ferulic acid esterase by microbial fermentation and its physical and chemical properties, structure, and classification. At the same time, the gene cloning of ferulic acid esterase from different sources and the application of ferulic acid esterase in food and medicine industries were reviewed.
Keywords:microorganism; ferulic acid esterase; exogenous expression
doi:10.3969/j.issn.1674-506X.2020.05-013
收稿日期:2020-02-26
基金项目:黑龙江省博士后科研启动基金项目(LBH-Q13139)
作者简介:李双双(1994-),女,硕士研究生。研究方向:微生物资源挖掘与利用。
*通讯作者
引文格式:李双双,赵辉.微生物阿魏酸酯酶性质及应用研究进展[J].食品与发酵科技,2020,56(5):75-80.